Journal: Advanced healthcare materials

Article Title: Designing Inherently Photodegradable Cell-Adhesive Hydrogels for 3D Cell Culture.

doi: 10.1002/adhm.202100632

Figure Lengend Snippet: Figure 2. A) Live/dead staining of normal human dermal fibroblasts (NHDF) with calcein-AM (green, live cells) and propidium iodide (red, dead cells) embedded in GelMA-free PEGMA/PEGDMA (Gel-N0) and GelMA-containing (Gel-N10) hydrogels on day 1, 7, 14 postencapsulation, followed by imaging with confocal microscopy (Leica TCS SPE, scale bar: 200 µm). B-i) NHDF embedded in Gel-N10 hydrogels on day 1 postencapsulation. Comparison of HepG2 cells encapsulated in (ii) Gel-N10 and (iii) Gel-N0 hydrogels (scale bar: 100 µm). C) Proliferation behavior of encapsulated HepG2 cells in Gel-N10 and Gel-N0 hydrogels over a period of 7 d assessed by PrestoBlue assay (n = 3). D) Toxicity evaluation of Gel-N10 photodegradation products by MTT proliferation assay with HeLa cells after 72 h exposure (n = 3). The concentration of nondiluted degradation products was 53 mg mL−1. Data are presented as mean ± SD and statistically evaluated by (C) Student’s t-test and (D) one-way ANOVA. *, **, *** represent p < 0.05, 0.01, and 0.001, respectively.

Article Snippet: Normal human dermal fibroblasts (NHDF), human liver cancer cells (HepG2), and HeLa cells were purchased from PromoCell GmbH (Heidelberg, Germany).

Techniques: Staining, Imaging, Confocal Microscopy, Comparison, Prestoblue Assay, Proliferation Assay, Concentration Assay

Journal: bioRxiv

Article Title: KRAB zinc finger proteins ZNF587/ZNF417 protect lymphoma cells from replicative stress-induced inflammation

doi: 10.1101/2023.03.08.531722

Figure Lengend Snippet: (a) Quantitative RT-PCR analysis of ZNF587B, ZNF814, ZNF587, ZNF417, and ZNF586 mRNAs 5 days after LV transduction with shRNAs targeting ZNF586, ZNF587B/ZNF814, and ZNF587/ZNF417 paralog pairs. Statistics: Student’s t-test. (b) Top: Bar plots showing the relative expression of ZNF586, ZNF417, ZNF587, ZNF587B, and ZNF814 in U2932 compared to OCI-Ly7 using GUSB, TBP, and/or ALAS2 housekeeping genes for normalization. Bottom: Bar plots showing the relative expression of ZNF587 and ZNF417 in SUDHL4, U2932, HBL1 cells compared to OCI-Ly7 using GUSB, TBP, and/or ALAS2 housekeeping genes for normalization. (c) MTT proliferation assays of U2932 and OCI-Ly7 upon LV transduction with two different anti-ZNF587/417 shRNAs, or control shRNA (shScr). Cells were plated after 5 days of LV transduction and 3 days of puromycin selection. (d) MTT proliferation assay of human primary dermal fibroblasts upon LV transduction with two anti-ZNF587/417 shRNAs or control shRNA. Cells were plated after 5 days of LV transduction and 3 days of puromycin selection. Quantitative RT-PCR analysis of ZNF587 and ZNF417 mRNAs 5 days after LV transduction. Statistics: Student’s t-test. (e) Scatter plot of RNA-seq from ZNF417/587 U2932 KD versus shScr control cells at day 3 of KD, outlining DEGs (grey dots, FDR <0.05) and among them genes belonging to the UV response UP Hallmark gene set (dark red dots) and MHC Class I genes (blue dots).

Article Snippet: Primary normal dermal fibroblasts were cultured in Fibroblast Basal Medium (ATCC) supplemented with Fibroblast Growth Kit-Low Serum (ATCC).

Techniques: Quantitative RT-PCR, Transduction, Expressing, Control, shRNA, Selection, Proliferation Assay, RNA Sequencing

Journal: Scientific Reports

Article Title: Effect of TAT-DOX-PEG irradiated gold nanoparticles conjugates on human osteosarcoma cells

doi: 10.1038/s41598-020-63245-8

Figure Lengend Snippet: Concentration dependence of cytotoxicity of carriers on ( a ) HOS cells and on ( b ) NHDF cells. Concentration dependence of cytotoxicity of carriers loaded with doxorubicin on ( c ) HOS cells and ( d ) NHDF cells.

Article Snippet: Human osteosarcoma (HOS) and Normal Human Dermal Fibroblasts (NHDF) cell lines were purchased from CLS Cell Lines Service GmbH and PromoCell, Eagle’s minimal essential medium (MEM) from Lonza, fetal bovine serum (FBS) from Biochrom GmbH, Germany and 1% Penicillin-Streptomycin-Amphotericin B mixture (10 K/10 K/25 µg in 100 ml) from Lonza, CellTiter 96® Aqueous One Solution Cell Proliferation Assay from Promega, Trypsin-Versene (EDTA) mixture from Lonza, phosphate buffered saline (PBS) from Invitrogen.

Techniques: Concentration Assay

Journal: Soft matter

Article Title: Encoding Cell-Instructive Cues to PEG-Based Hydrogels via Triple Helical Peptide Assembly

doi: 10.1039/C2SM25903F

Figure Lengend Snippet: Phase contrast micrographs of human dermal neonatal fibroblasts seeded onto PEGDA-CMP scaffolds modified with CMP-RGD (A), PEGDA-CMP modified with CMP (B), and PEGDA modified with CMP-RGD (C).

Article Snippet: Fibroblasts (human dermal, neonatal) were obtained from American Type Culture Collection (Manassas, VA), and DMEM/F12+GlutaMAX media was obtained from Invitrogen.

Techniques: Modification

Journal: Soft matter

Article Title: Encoding Cell-Instructive Cues to PEG-Based Hydrogels via Triple Helical Peptide Assembly

doi: 10.1039/C2SM25903F

Figure Lengend Snippet: WST-1 proliferation assay (absorbance at 440 nm) of fibroblasts seeded on PEGDA-CMP modified with CMP-RGD and control hydrogels: PEGDA-CMP modified with CMP and PEGDA hydrogel (without CMP) modified with CMP-RGD. CMP-RGD modified PEGDA-CMP scaffolds showed higher cell proliferation compared to control samples (Student’s t test, p < 0.005). Data reported as mean ± SD.

Article Snippet: Fibroblasts (human dermal, neonatal) were obtained from American Type Culture Collection (Manassas, VA), and DMEM/F12+GlutaMAX media was obtained from Invitrogen.

Techniques: Proliferation Assay, Modification, Control

Journal: Soft matter

Article Title: Encoding Cell-Instructive Cues to PEG-Based Hydrogels via Triple Helical Peptide Assembly

doi: 10.1039/C2SM25903F

Figure Lengend Snippet: A) Fluorescence micrographs and corresponding cell area histograms of fibroblasts stained with DAPI (stains nucleus in blue) and phalloidin (stains actin in green). Cells exhibited enhanced adhesion and spread morphology on PEGDA-CMP scaffolds modified with increasing concentrations of CMP-RGD. B) Cell area histograms for cells seeded on control scaffolds showing reduced cell adhesion. Data reported as mean ± SD.

Article Snippet: Fibroblasts (human dermal, neonatal) were obtained from American Type Culture Collection (Manassas, VA), and DMEM/F12+GlutaMAX media was obtained from Invitrogen.

Techniques: Fluorescence, Staining, Modification, Control

Journal: Soft matter

Article Title: Encoding Cell-Instructive Cues to PEG-Based Hydrogels via Triple Helical Peptide Assembly

doi: 10.1039/C2SM25903F

Figure Lengend Snippet: A) Image of PEGDA-CMP gel partially treated with CF-CMP-RGD and CF absorbance profile across the gel. B) Cell area histograms (fibroblasts after 24 h culture) corresponding to the three areas defined in A demonstrating modulation of cell morphology across PEGDA-CMP scaffolds with RGD gradient formed by triple helical CMP association. Data reported as mean ± SD.

Article Snippet: Fibroblasts (human dermal, neonatal) were obtained from American Type Culture Collection (Manassas, VA), and DMEM/F12+GlutaMAX media was obtained from Invitrogen.

Techniques: